Impact of Drying Conditions on Antioxidant Activity of Red Clover (Trifolium pratense), Sweet Violet (Viola odorata) and Elderberry Flowers (Sambucus nigra).

Flowers of red clover (Trifolium pratense), sweet violet (Viola odorata) and elderflowers (Sambucus nigra) were dried by means of air drying at 30 °C and 50 °C and by freeze drying. The content of polyphenols was determined using the Folin-Ciocalteu reagent, while anthocyanins were quantified by the pH differential method. Antioxidant activities of aqueous and ethanolic extracts of the dried flowers were measured by the DPPH and ABTS assays, as well as FRAP and reducing power methods. The highest amount of polyphenols was determined in the ethanolic extracts of fresh red clover flowers (854.76 mg/100 g), while the highest concentration of anthocyanins was determined in the aqueous extracts of fresh sweet violet flowers (99.41 mg/100 g). The results showed that, in general, the extracts of red clover flower were characterized by the highest antioxidant activity, while the sweet violet extracts had the poorest antioxidant properties, although these values fluctuated depending on the method used. There was strong correlation between antioxidant activity and TPC (r = 0.9196, FRAP method). In most cases, freeze drying was found to be the best conservation method, retaining well the antioxidant properties of the tested flowers and the compounds determining these properties.

The Influence of Organic Vanadium Complexes on an Antioxidant Profile in Adipose Tissue in Wistar Rats.

One of the aspects of biological activity of vanadium is its influence on carbohydrate metabolism. For more than 30 years, various vanadium complexes have been tested as antidiabetic agents. This study researched organic vanadium complexes with bipyridinium ligands and their influences on metabolic rate, as well as on the antioxidant activity of adipose tissue. The effects of sodium (2,2'-bipyridine) oxidobisperoxovanadate (V) octahydrate (known as the V complex), bis(2,2'-bipyridine) oxidovanadium (IV) sulfate dehydrate (known as the B complex), and bis(4.4'-dimethyl-2,2'-bipyridine) oxidovanadium (IV) sulfate dihydrate (labelled as the BM complex) were assessed. Solutions of the tested complexes were introduced intraperitoneally with a probe to animals fed with either a control diet or a high-fat diet. The BM complex had a significant influence on the increase in ferric reducing antioxidant power, as well as on the concentration of glutathione in the adipose tissue of rats fed with a high-fat diet. The V complex increased the concentration of glutathione in the adipose tissue of rats fed with control fodder, as well as significantly reduced the relative change in rat weight for the high-fat diet. Furthermore, the presence of each tested vanadium complex had an impact of statistically significant increase in basal metabolic rate, regardless of applied diet. Further research on these organic vanadium complexes is necessary to understand the mechanisms responsible for their ability to affect adipose tissue.

Sustainable Drying and Torrefaction Processes of Miscanthus for Use as a Pelletized Solid Biofuel and Biocarbon-Carrier for Fertilizers.

Miscanthus is resistant to dry, frosty winters in Poland and most European Union countries. Miscanthus gives higher yields compared to native species. Farmers can produce Miscanthus pellets after drying it for their own heating purposes. From the third year, the most efficient plant development begins, resulting in a yield of 25-30 tons of dry matter from an area of 1 hectare. Laboratory scale tests were carried out on the processes of drying, compacting, and torrefaction of this biomass type. The analysis of the drying process was conducted at three temperature levels of the drying agent (60, 100, and 140 °C). Compaction on a hydraulic press was carried out in the pressure range characteristic of a pressure agglomeration (130.8-457.8 MPa) at different moisture contents of the raw material (0.5% and 10%). The main interest in this part was to assess the influence of drying temperature, moisture content, and compaction pressure on the specific densities (DE) and the mechanical durability of the pellets (DU). In the next step, laboratory analyses of the torrefaction process were carried out, initially using the Thermogravimetric Analysis TGA and Differential Scaning Calorimeter DSC techniques (to assess activation energy (EA)), followed by a flow reactor operating at five temperature levels (225, 250, 275, 300, and 525 °C). A SEM analysis of Miscanthus after torrefaction processes at three different temperatures was performed. Both the parameters of biochar (proximate and ultimate analysis) and the quality of the torgas (volatile organic content (VOC)) were analyzed. The results show that both drying temperature and moisture level will affect the quality of the pellets. Analysis of the torrefaction process shows clearly that the optimum process temperature would be around 300-340 °C from a mass loss ratio and economical perspective.

Effect of Compaction Pressure and Moisture Content on Post-Agglomeration Elastic Springback of Pellets.

Renewable energy sources (RES) represent an increasing share of global energy production. Biomass has the highest potential of all RES. Biomass is used to produce solid biofuels, liquid biofuels, and gaseous biofuels. One of the main directions of research on solid biofuels is to optimize the agglomeration process. The main factors determining the characteristics of the final product in the production of pellets are process and material parameters. Process parameters include compaction pressure, temperature, and geometry of the matrix channel. The parameters of the material are the type of biomass, moisture content, degree of fragmentation, and method of preparation of the material (e.g., drying). The process of pressure compaction is always associated with the negative phenomenon of elastic springback. The aim of this work was to check the influence of compaction pressure and material moisture content on the springback value. The research was conducted on three materials (giant miscanthus, cup plant and Virginia mallow), using four different pressures (131, 196, 262, and 327 MPa) and three different moisture levels (8, 11, and 14%). For all material springback values, the range was 9-16%. Statistical analysis showed that for all plants tested, the effects of compaction pressure and moisture content significantly affected the elastic springback value. Areas of high value springback in the pattern of process parameters were determined.

Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves.

The influence of harvest period and drying method of Salvia officinalis L. leaves on the content of essential oils, polyphenols and antioxidant properties was investigated. Sage leaves were collected twice during plant blossoming (in June and July) and dried traditionally in natural conditions and at 35 °C. Antioxidant activity was assayed in methanol-acetone extracts and infusions of dried leaves with the use of free radical scavenging activity (DPPH) and ferric reducing antioxidant power (FRAP) technique. Total phenolic content in extracts as well as in infusions was determined by the means of Folin-Ciocalteu method. Based on the LC/MS analysis, the polyphenol compounds present in both extracts and in infusions were identified. The extracts contained more polyphenols and were characterized by higher antioxidant activity than infusions. In the extracts significant amount of ferulic acid was found, which was influenced both by the harvest period and drying method. The amount of ferulic acid found in extracts obtained from the June leaves dried traditionally was three times lower (6.185 µg/g DW) than in extracts from July leaves dried in the same conditions. Harvest period had a significant effect on the essential oils' content, leaves collected in July contained 15% more oils than those collected in June.